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 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
22/11/2002 |
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Data da última atualização: |
20/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COUTINHO, H. L. da C.; KAY, H. E.; MANFIO, G. P.; NEVES, M. C. P.; RIBEIRO, J. R. A.; RUMJANEK, N. G.; BERINGER, J. E. |
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Afiliação: |
HEITOR LUIZ DA COSTA COUTINHO, CNPS; HAZEL E. KAY, UNIVERSITY OF BRISTOL; GILSON PAULO MANFIO, UNIVERSITY OF NEWCASTLE; MARIA CRISTINA PRATA NEVES, CNPAB; JOSÉ ROBERTO A. RIBEIRO, CNPAB; NORMA GOUVEA RUMJANEK, CNPAB; JOHN E. BERINGER, UNIVERSITY OF BRISTOL. |
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Título: |
Molecular evidence for shifts in polysaccharide composition associated with adaptation of soybean Bradyrhizobium strains to the Brazilian Cerrado soils |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Environmental Microbiology, v. 1, n. 5, p. 401-408, Sept. 1999. |
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DOI: |
https://doi.org/10.1046/j.1462-2920.1999.00047.x |
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Idioma: |
Inglês |
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Conteúdo: |
Pyrolysis mass spectrometry (PyMS) and DNA fingerprinting (RAPD and RSa hybridization) were used to characterize soybean inoculant strains and root nodule isolates of bradyrhizobia from the Brazilian Cerrado soils. Most isolates were shown to be derived from the inoculant strains on the basis of genotype comparisons by DNA fingerprinting. Phenotypic analysis (using PyMS) of the strains and separately of the polysaccharides derived from them showed that the nodule isolates differed from the parental strains, suggesting adaptation to the Cerrado soil environment. The extent of the differences between the derivatives and inoculant strains was similar for comparisons made on the basis of whole-cell preparations or from the isolated polysaccharides, indicating that the adaptation was caused by changes in the composition of the polysaccharides produced. |
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Thesagro: |
Cerrado; Soja; Solo. |
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Thesaurus Nal: |
DNA fingerprinting; Mass spectrometry; Pyrolysis. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 01746naa a2200277 a 4500
001 1336379
005 2021-09-20
008 1999 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1046/j.1462-2920.1999.00047.x$2DOI
100 1 $aCOUTINHO, H. L. da C.
245 $aMolecular evidence for shifts in polysaccharide composition associated with adaptation of soybean Bradyrhizobium strains to the Brazilian Cerrado soils$h[electronic resource]
260 $c1999
520 $aPyrolysis mass spectrometry (PyMS) and DNA fingerprinting (RAPD and RSa hybridization) were used to characterize soybean inoculant strains and root nodule isolates of bradyrhizobia from the Brazilian Cerrado soils. Most isolates were shown to be derived from the inoculant strains on the basis of genotype comparisons by DNA fingerprinting. Phenotypic analysis (using PyMS) of the strains and separately of the polysaccharides derived from them showed that the nodule isolates differed from the parental strains, suggesting adaptation to the Cerrado soil environment. The extent of the differences between the derivatives and inoculant strains was similar for comparisons made on the basis of whole-cell preparations or from the isolated polysaccharides, indicating that the adaptation was caused by changes in the composition of the polysaccharides produced.
650 $aDNA fingerprinting
650 $aMass spectrometry
650 $aPyrolysis
650 $aCerrado
650 $aSoja
650 $aSolo
700 1 $aKAY, H. E.
700 1 $aMANFIO, G. P.
700 1 $aNEVES, M. C. P.
700 1 $aRIBEIRO, J. R. A.
700 1 $aRUMJANEK, N. G.
700 1 $aBERINGER, J. E.
773 $tEnvironmental Microbiology$gv. 1, n. 5, p. 401-408, Sept. 1999.
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Registro original: |
Embrapa Solos (CNPS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/09/2013 |
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Data da última atualização: |
19/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
GARITA, L. C.; TASSI, A. D.; CALEGARIO, R. F.; KITAJIMA, E. W.; CARBONELL, S. A. M.; ASTUA, J. de F. |
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Afiliação: |
L. C. GARITA, ESALQ; A. D. TASSI, ESALQ; R. F. CALEGARIO, ESALQ; E. W. KITAJIMA, ESALQ; S. A. M. CARBONELL, IAC; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Common bean: experimental indicator plant for Citrus leprosis virus C and some other Cytoplasmic-type brevipalpus-transmitted viruses. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Plant Disease, v. 97, n. 10, p. 1346, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus leprosis (CL) caused by Citrus leprosis virus C (CiLV-C) is present in Latin America from Mexico to Argentina, where citrus plants are grown. CiLV-C is transmitted by the tenuipalpid mite, Brevipalpus phoenicis, causing localized lesions on citrus leaves, fruit, and stems. One limitation to study of the virus vector host relationship in this pathosystem is the lack of a suitable assay plant. On Citrus spp. used as susceptible hosts, symptoms may take weeks or months to appear after experimental inoculation by viruliferous mites. Common bean (Phaseolus vulgaris) was found to respond with localized necrotic lesions after inoculation with viruliferous B. phoenicis in 5 days. Thus far, 113 tested common bean varieties and lines and some recent accessions of varied genetic background behaved in a similar way. Black bean IAC Una was adopted as a standard test variety. When inoculated leaves were left at 28 to 30°C, the period for the lesion appearance was reduced to only 2 days. Confirmation that the lesions on common bean leaves are caused by CiLV-C were made by transmission electron microscopy, immunofluorescence, enzyme-linked immunosorbent assay, and reverse-transcription polymerase chain reaction specific for CiLV-C. Common bean plants mite-inoculated with some other cytoplasmic-type Brevipalpus-transmitted viruses (BrTVs) (Passion fruit green spot virus, Solanum violaefolium ringspot virus, Ligustrum ringspot virus, and Hibiscus green spot virus) also responded with necrotic local lesions and may serve as test plants for these viruses. Two nuclear types of BrTV (Coffee ringspot virus and Clerodendrum chlorotic spot virus) were unable to produce symptoms on common bean. MenosCitrus leprosis (CL) caused by Citrus leprosis virus C (CiLV-C) is present in Latin America from Mexico to Argentina, where citrus plants are grown. CiLV-C is transmitted by the tenuipalpid mite, Brevipalpus phoenicis, causing localized lesions on citrus leaves, fruit, and stems. One limitation to study of the virus vector host relationship in this pathosystem is the lack of a suitable assay plant. On Citrus spp. used as susceptible hosts, symptoms may take weeks or months to appear after experimental inoculation by viruliferous mites. Common bean (Phaseolus vulgaris) was found to respond with localized necrotic lesions after inoculation with viruliferous B. phoenicis in 5 days. Thus far, 113 tested common bean varieties and lines and some recent accessions of varied genetic background behaved in a similar way. Black bean IAC Una was adopted as a standard test variety. When inoculated leaves were left at 28 to 30°C, the period for the lesion appearance was reduced to only 2 days. Confirmation that the lesions on common bean leaves are caused by CiLV-C were made by transmission electron microscopy, immunofluorescence, enzyme-linked immunosorbent assay, and reverse-transcription polymerase chain reaction specific for CiLV-C. Common bean plants mite-inoculated with some other cytoplasmic-type Brevipalpus-transmitted viruses (BrTVs) (Passion fruit green spot virus, Solanum violaefolium ringspot virus, Ligustrum ringspot virus, and Hibiscus green spot virus) also responded with n... Mostrar Tudo |
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Thesagro: |
Brevipalpus Phoenicis; Phaseolus Vulgaris. |
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Thesaurus NAL: |
Beans; Citrus leprosis virus C. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02426naa a2200229 a 4500
001 1966750
005 2023-05-19
008 2013 bl uuuu u00u1 u #d
100 1 $aGARITA, L. C.
245 $aCommon bean$bexperimental indicator plant for Citrus leprosis virus C and some other Cytoplasmic-type brevipalpus-transmitted viruses.$h[electronic resource]
260 $c2013
520 $aCitrus leprosis (CL) caused by Citrus leprosis virus C (CiLV-C) is present in Latin America from Mexico to Argentina, where citrus plants are grown. CiLV-C is transmitted by the tenuipalpid mite, Brevipalpus phoenicis, causing localized lesions on citrus leaves, fruit, and stems. One limitation to study of the virus vector host relationship in this pathosystem is the lack of a suitable assay plant. On Citrus spp. used as susceptible hosts, symptoms may take weeks or months to appear after experimental inoculation by viruliferous mites. Common bean (Phaseolus vulgaris) was found to respond with localized necrotic lesions after inoculation with viruliferous B. phoenicis in 5 days. Thus far, 113 tested common bean varieties and lines and some recent accessions of varied genetic background behaved in a similar way. Black bean IAC Una was adopted as a standard test variety. When inoculated leaves were left at 28 to 30°C, the period for the lesion appearance was reduced to only 2 days. Confirmation that the lesions on common bean leaves are caused by CiLV-C were made by transmission electron microscopy, immunofluorescence, enzyme-linked immunosorbent assay, and reverse-transcription polymerase chain reaction specific for CiLV-C. Common bean plants mite-inoculated with some other cytoplasmic-type Brevipalpus-transmitted viruses (BrTVs) (Passion fruit green spot virus, Solanum violaefolium ringspot virus, Ligustrum ringspot virus, and Hibiscus green spot virus) also responded with necrotic local lesions and may serve as test plants for these viruses. Two nuclear types of BrTV (Coffee ringspot virus and Clerodendrum chlorotic spot virus) were unable to produce symptoms on common bean.
650 $aBeans
650 $aCitrus leprosis virus C
650 $aBrevipalpus Phoenicis
650 $aPhaseolus Vulgaris
700 1 $aTASSI, A. D.
700 1 $aCALEGARIO, R. F.
700 1 $aKITAJIMA, E. W.
700 1 $aCARBONELL, S. A. M.
700 1 $aASTUA, J. de F.
773 $tPlant Disease$gv. 97, n. 10, p. 1346, 2013.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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